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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
TEM-132-producing Ceftatidime-resistant Klebsiella pneumoniae That Cause a Probable Healthcare Associated Transmission |
| Language |
J |
| AuthorList |
Maho Takahashi1), Naohiro Shibata2), Kimiko Ooya1), Misako Horimizu1), Kazuhiko Nagai3), Yoshichika Arakawa2) |
| Affiliation |
1) Department of Clinical Laboratory, kaetsu Hospital, Niigata, Japan
2) Department of Bacterial Pathogenic and Infection Control, National Instiute of Infection Deseases
3) Department of Pharmacy, Kaetsu Hospital, Niigata, Japan |
| Publication |
J.J.C.M.: 21 (1), 11-16, 2011 |
| Received |
June 27, 2007 |
| Accepted |
October 20, 2010 |
| Abstract |
Ceftazidime (CAZ)-resistant Klebsiella pneumoniae strains were isolated from 95 inpatients during April, 2003 and November, 2004. To characterize the molecular mechanisms of the CAZ-resistance, PCR detection of bla genes, nucleotide sequencing of the PCR products were performed for the 21 isolates, as well as pulsed-field electrophoresis (PFGE). All isolates were found to harbor blaTEM-132 by PCR amplification and nucleotide sequence analyses. By PFGE analysis, 19 isolates demonstrated the same PFGE profile suggesting a probable healthcare associated transmission of TEM-132-producing K. pneumoniae. To cope with this outbreak, the contact precaution, together with the standard precaution and enhanced sanitation of hospital environment, was performed, and we finally succeeded in the prevention of further transmission of the CAZ-resistant K. pneumoniae. Most ESBLs found in CAZ-resistant K. pneumoniae have been SHV-derived ESBLs, but the enzyme identified in the CAZ-resistant K. pneumoniae was TEM-132 that has never been reported in Japan. |
| Keywords |
Klebsiella pneumoiae |
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