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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Reduction of Inoculums in Commercial Transport Swabs for Bordetella pertussis Transport |
| Language |
J |
| AuthorList |
Junpei Okada, Masaru Komatsu, Hiroto Tanaka, Kiyoko Katoh, Kimika Murakami, Emu Fujiwara, Koji Matsuoka, Takuya Maede |
| Affiliation |
Central Laboratory Technical Section 3, FALCO Biosystems Ltd.,
17-1 Tai-nishiarami Kumiyama-cho, Kuze-gun, Kyoto 613-0036, Japan |
| Publication |
J.J.C.M.: 21 (2), 129-133, 2011 |
| Received |
November 7, 2010 |
| Accepted |
January 26, 2011 |
| Abstract |
We investigated reduction of inoculums of Bordetella pertussis in each commercial transport swabs. Transport media were chosen as following; (1) Seed swab γI (Eiken Chemical, Ltd., Cary-Blair media), (2) Seed swab γIII (Eiken Chemical, Ltd., Amies with charcoal), (3) BD BBL Culture Swab Plus Single (BD, Amies), (4) BD BBL Culture Swab EZ (BD, additive-free of media) and (5) Mentip (JCB Industry Ltd., the swab was kept in a sterilized spitz). There was inoculums size from 3.2 to 13.5% of Bordetella pertussis ATCC9797 in each commercial transport swabs. Reduce of inoculums size was from 0 to 70.3% for 24 hours of the room temperature storage. (3) and (5) in particular remarkable reduced inoculums size. We demonstrated that all of five commercial swabs were hard to take inoculums, inoculums sizes were remarkably decrease caused by storage, and it might be difficult to perform Bordetella pertussis culture by the swab method. |
| Keywords |
Bordetella pertussis |
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