Journal

The Journal of the Japanese Society for Clinical Microbiology

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[Vol.21 No.2 contents]
Japanese / English

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Article in Japanese

ArticleTitle A Case of Osteomyelitis Caused by Mycobacterium farcinogenes
Language J
AuthorList Masahiro Isozaki1), Yu Kaneko1), Kumiko Matsushita1), Kiyofumi Ohkusu2)
Affiliation 1) Department of Clinical Laboratory, Amakusa Medical Center
2) Department of Microbiology, Gifu University Graduate School of Medicine
Publication J.J.C.M.: 21 (2), 134-137, 2011
Received August 20, 2010
Accepted March 2, 2011
Abstract The patient was a 66-year-old male who had fallen off a bicycle while cycling down a hill on January 1st, 2008. He underwent osteosynthesis for a compound fracture in the left lower thigh. Since swelling and pain occurred in the vicinity of the lesion around April 30th and infection was suspected, he visited the Orthopedic Department of our hospital and was hospitalized for treatment, including surgery. Pus was collected for culturing during incisional purulent drainage of the swelling lesion. On the second day of culturing, Corynebacterium-like opaque white colonies were observed. Weak Gram-positive bacillus was found and was suspected to be an acid-fast bacterium. However, a mixture of acid-fast and non-acid-fast bacteria was observed by Kinyoun staining, which made it difficult to identify the colonies as acid-fast bacterium or Gram-positive bacillus. Based on the difficulty of identifying the bacteria and the pathology of osteomyelitis, a genetic analysis was conducted. This analysis of the base sequences of the 16S rDNA and ITS regions confirmed the presence of M. farcinogenes. This is the first case report of an infectious disease involving M. farcinogenes in Japan.
Keywords Mycobacterium farcinogenes, 16S rDNA
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