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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Detection of AAC(6´)-Iae Producing Strain in Pseudomonas aeruginosa |
| Language |
J |
| AuthorList |
Masahiro Shimojima1), Kazuhiro Sakairi1), Miho Ogawa1), Toru Akiyama2), Masashi Tanaka3), Kenji Naraharai3), Teruo Kirikae2), Mituo Kaku4) |
| Affiliation |
1) Department of Microbiology, BML,Inc.
2) Department of Infectious Diseases, Research Institute, National Center for Global and Medicine
3) Mizuho Medy Co., Ltd., R&D
4) Department of Infection Control and Laboratory Diagnostics, Internal Medicine, Tohoku University Graduate School of Medicine |
| Publication |
J.J.C.M.: 21 (4), 247-252, 2011 |
| Received |
April 6, 2011 |
| Accepted |
September 5, 2011 |
| Abstract |
Various medical institutions within the nation have commissioned our company to conduct microbial tests, and we conduct susceptibility tests for each type of antibacterial drug. As such, we can obtain an understanding of incidence trend of drug-resistant bacteria, and we have been reporting drug susceptibility for each type of antibacterial drug in fresh clinical isolates discovered in each prefecture every year starting October 2001 in order to contribute to the transmission of information. Among these isolates, incidence of multidrug-resistant Pseudomonas aeruginosa (MDRP) has shifted from 0.58% in April 2007-March 2008 (692/120,003 strains)1) to 3.88% in April 2008-March 2009 (5,012/129,274 strains)2) and to 4.06% in April 2009-March 2010 (5,499/135,463 strains).3) This time, we were provided the opportunity to use "AAC(6´)-Iae Chromat" (Mizuho Medy Co., Ltd.)4) that was developed for the purpose to quickly and specifically detect aminoglycoside acetyltransferase AAC(6´)-Iae, which is estimated to be found in at least half of all MDRP, using an easy method; therefore, we attempted to detect AAC(6´)-Iae among the 297 clinical isolate strains of Pseudomonas aeruginosa that showed drug resistance in the drug susceptibility tests. P. aeruginosa strains that possess the genes which code for AAC(6´)-Iae have been confirmed through molecular biological analysis of isolate strains from medical institutions which have reported high incidence of MDRP.5), 7) Of the 254 strains of MDRP picked up in our company, 152 strains (59.8%) were confirmed to possess AAC(6´)-Iae. In addition, it has also become apparent that these strains are dispersed throughout the nation. Of the 297 drug resistant P. aeruginosa strains including MDRP chosen for this study, 34 strains (11.4%) were severe MDPR, or strains which are considered important to focus on when considering measures against nosocomial infections, that showed high MIC levels (i.e. 128 ug/ml or more against the following three drugs: IPM, AMK, and CPFX); and, of these strains, 31 strains (91.2%), a very high percentage, possessed AAC(6´)-Iae. As a result, by using "AAC(6´)-Iae Chromat," it was possible to easily identify within 15 minutes which strains were severe MDRP that produce AAC-Iae. Based on this, the product demonstrated that it was a useful kit for measures against nosocomial infections where promptness is considered important. |
| Keywords |
MDRP |
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