 |
The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Comparison of Broth Microdilution Method with the E-Test for Candida spp. Susceptibility to Antifungal Agents |
| Language |
J |
| AuthorList |
Hiromi Morishige1), Yoko Mano2), Mieko Goto3), Toyoko Oguri4), Nobuhiko Furuya1,2) |
| Affiliation |
1) Graduate School of Health Care Science, Bunkyo Gakuin University
2) Faculty of Health Science Technology, Bunkyo Gakuin University
3) The University of Tokyo Hospital
4) Clinical Laboratory, Kameda Medical Center |
| Publication |
J.J.C.M.: 22 (2), 112-119, 2012 |
| Received |
December 1, 2011 |
| Accepted |
February 10, 2012 |
| Abstract |
Recently, the increase in incidence of deep mycoses and the occurrence of fluconazole (FLCZ)-resistant Candida albicans require the accurate determination of MIC against fungi. We compared and evaluated the MICs of antifungal agents by broth microdilution based on CLSI M27-A3 and E-test. In this study, we tested isolates from each of the following species: reference isolates (7 isolates), C. albicans (36 isolates), Candida glabrata (20 isolates), and Candida tropicalis (17 isolates). These methods were used to determine the MICs of amphotericin B (AMPH-B), flucytosine (5-FC), itraconazole (ITCZ), FLCZ for all 80 isolates. The rates of MIC agreement within 2 fold dilution between broth microdilution and E-test methods were as follows: 5-FC, 92.5%; FLCZ, 72.6%; ITCZ, 65.0%. On the other hand, MICs of E-test were lower more than 4 fold dilution than those of broth microdilution in AMPH-B (96.3%), ITCZ (27.5%) and FLCZ (25.0%). Several isolates showed discrepancies of susceptibility according to methods, strains and/or agents except for C. glabrata and C. tropicalis for 5-FC and C. tropicalis for FLCZ. In this study, the MICs of E-test tended to be lower than broth microdilution method against many isolates tested. Therefore, these results suggest that interpretation of susceptibility required the special attention. |
| Keywords |
Candida, CLSI, broth microdilution, E-test, MIC |
|
