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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Antimicrobial Susceptibility Testing for Corynebacterium spp. and Erysipelothrix rhusiopathiae |
| Language |
J |
| AuthorList |
Yoshihito Otsuka1), Akihiro Toguchi1), Hidetaka Kitazono2), Naoto Hosokawa2), Asuka Kamioka3), Toyoko Oguri1) |
| Affiliation |
|
| Publication |
J.J.C.M.: 22 (2), 120-125, 2012 |
| Received |
January 6, 2012 |
| Accepted |
March 27, 2012 |
| Abstract |
Corynebacterium spp. and Erysipelothrix rhusiopathiae susceptibility test is currently performed using 2.5-5.0%v/v lysed horse blood at 35°C as an adjuvant to Mueller-Hinton broth, which is recommended by the Clinical and Laboratory Standards Institute(CLSI). We evaluated the usefulness of Strepto-Haemo Supplement "Eiken®" (SHS) contains 1.5 ml of lysed horse blood, 1.07 mg of beta-NAD, and 0.36 g of yeast extract, compared with the current standard lysed horse blood, as an adjuvant to Mueller-Hinton broth for the Corynebacterium spp. and E. rhusiopathiae susceptibility test. For all 58 clinical isolates of Corynebacterium spp. and E. rhusiopathiae tested, the SHS method and the standard CLSI method showed almost identical MIC results within one dilution difference. We believe that the SHS can be an alternative to the currently recommended lysed horse blood to test Corynebacterium spp. and E. rhusiopathiae susceptibility. |
| Keywords |
Corynebacterium |
|
