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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Development of New Screening Medium to Detect Multidrug-Resistant Pseudomonas aeruginosa for Increased Efficiency of Infection Control |
| Language |
J |
| AuthorList |
Satoru Yokoyama1), Kumiko Kawamura1), Tetsuya Yagi2), Yoshichika Arakawa3,4) |
| Affiliation |
1) Department of Pathophysiological Laboratory Science, Nagoya University Graduate School of Medicine
2) Department of Infectious Diseases, Nagoya University Graduate School of Medicine
3) Department of Bacteriology, Nagoya University Graduate School of Medicine
4) Department of Bacteriology II, National Institute of Infectious Diseases |
| Publication |
J.J.C.M.: 22 (2), 126-134, 2012 |
| Received |
November 25, 2011 |
| Accepted |
May 1, 2012 |
| Abstract |
Multidrug-resistant Pseudomonas aeruginosa (MDRP) is responsible for severe nosocomial infections, and rapid and accurate methods to detect strains of this bacterium are needed. We developed a new MDRP screening medium by incorporating three antimicrobial agents (amikacin (AMK), imipenem/cilastatin (IPM) and ciprofloxacin (CPFX)) into an existing medium for the detection of Pseudomonas species, CHROMagarTM Pseudomonas (CHROMagar). A panel of 138 Gram-negative bacteria (mainly Pseudomonas sp., Acinetobacter sp., Escherichia coli, Klebsiella sp. and Serratia sp.) harboring a variety of β-lactamase genes were assembled. These bacteria were then used for the purpose of evaluating MDRP screening medium with the addition of either 4 or 6 μg/ml IPM, 1 μg/ml AMK and 1 μg/ml of CPFX. Of 47 strains of MDRP, isolates harboring blaIMP or blaVIM genes grew specifically on MDRP screening medium, while the other non-β-lactamase-producing MDRP isolates did not grow. This suggests the contribution of a carbapemen-resistant mechanism to the system's ability to detect MDRP. Serratia marcescens harboring the blaIMP gene and Klebsiella pneumoniae harboring the blaKPC gene grew on the MDRP screening medium, although they were easily distinguishable from Pseudomonas sp. (blue colonies) by their typical colony color (white colonies). Based on these results, we determined that CHROMagar with 1 μg/ml of AMK, 4 μg/ml of IPM and 1 μg/ml of CPFX showed the best detection limit (103 CFU/ml), sensitivity (80.9%), specificity (96.7%), and stability. This MDRP screening medium is a simple, rapid and cost-effective method compared with conventional methods described in the literature. The use of this MDRP screening medium may make it possible to efficiently detect MDRP isolates in clinical material for infection control. |
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