 |
The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Comparison of Three Mediums for Detection of Methicillin-Resistant Staphylococcus aureus |
| Language |
J |
| AuthorList |
Keigo Kimura1), Masahiro Toyokawa1), Isao Nishi1), Junko Sunada1), Akiko Ueda1), Tomomi Sakata1), Yoriko Inoue1), Seishi Asari2) |
| Affiliation |
1) Laboratory for Clinical Investigation, Osaka University Hospital
2) Department of Infection and Pervention, Osaka University Hospital |
| Publication |
J.J.C.M.: 22 (4), 255-262, 2012 |
| Received |
April 19, 2012 |
| Accepted |
September 18, 2012 |
| Abstract |
We evaluated Brilliance MRSA2 (Brilliance), CHROMagar MRSA (CHROM), and MRDS-K media for the detection of methicillin-resistant Staphylococcus aureus (MRSA). In this study, 610 nasal swab specimens were used. A true positive result was defined as the presence of MRSA on at least one medium and a true negative result was defined as no growth on any media. After 24 h of incubation, the sensitivity, specificity, positive predictive values (PPV), and negative predictive values (NPV) were 85.5%, 99.7%, 96.4%, and 98.8% for Brilliance; 87.1%, 100%, 100%, and 98.9% for CHROM; and 85.5%, 100%, 100%, and 98.8% for MDRS-K, respectively. Conversely, after 48 h, we detected MRSA from 1 specimen on Brilliance, 2 specimens on CHROM, and 4 specimens on MDRS-K. The sensitivity of all three media was high, and we confirmed the growth of methicillin-sensitive S. aureus from 11 specimens on Brilliance and 1 specimen on CHROM. (At 48 h, the sensitivity/specificity/PPV/NPV for Brilliance; 87.1%/98.5%/83.1%/98.9%, CHROM; 90.3%/99.9%/98.2%/99.2%, MDRS-K; 91.9%/100%/100%/99.3%). When we tested 2 borderline MRSA strains and 2 small colony variant (SCV) MRSA strains, 3 strains except for 1 SCV MRSA strain were detected by all 3 mediums as well. In conclusion, it is important that we analyze the characteristics of each MRSA detection mediums and use each appropriately to maintain high sensitivity and specificity. |
| Keywords |
Methicillin-resistant Staphylococcus aureus (MRSA), Brilliance MRSA2, CHROMagar MRSA, MDRS-K |
|
