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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
A Novel Method of Detection of H7 Flagella Antigen of Enterohemorrhagic Escherichia coli O157 by Using Motility Enhancement Medium Reinforced by Means of Prolonged Autoclave Treatment |
| Language |
J |
| AuthorList |
Hidemi Aoki1)*, Mitsugu Yamazaki2)*, Tomoko Hayashi1), Norio Tatsumi3), Satoru Hattori1) *Co-first authors |
| Affiliation |
1) Aichi Prefectural Kinuura-Tobu Health Center
2) Aichi Prefectural Ichinomiya Health Center
3) Anjo Kosei Hospital |
| Publication |
J.J.C.M.: 22 (4), 272-278, 2012 |
| Received |
August 3, 2012 |
| Accepted |
October 16, 2012 |
| Abstract |
We have found a phenomenon to increase motility of enterohemorrhagic Escherichia coli O157 (EHEC O157) by culturing in Brain Heart Infusion (BHI) medium autoclaved for 120 minutes. Based on the phenomenon, we developed an accurate and rapid method for detecting H7 flagellar antigen of EHEC O157. Before an agglutination test of H7 antigen, it usually performed multiple passages of EHEC O157 in motility enhancement medium, which into the Craigie's tube was inserted. The method described here does not require the troublesome multiple passages, because it uses the motility enhancement medium reinforced. The EHEC O157 was inoculated in Brain Heart Infusion medium, which were autoclaved at 121°C for 120 minutes. The broth cultures used for the H7 agglutination test are obtained at incubation at 37°C for 6 or 24 hours. The rapid method was able to classify 85 (97%) out of the 88 EHEC O157 strains which were H7-typed by conventional method within 2 days. The three strains, not H7-typed by the rapid method, had very weak motility, so that required more than 6 passages (more than 2 weeks) to gain enough motility for H7-typing by the conventional method. The procedure here significantly shortens the period of H7-typing of EHEC O157 (approximately 3 to 5 days), and could be adapted easily for routine use in clinical laboratories. |
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