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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
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[Vol.22 No.4 contents] Japanese / English |
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| ArticleTitle | External Quality Assessment for Direct Acid-Fast Bacilli Smear Microscopy |
| Language | J |
| AuthorList | Akio Aono1), Kinuyo Chikamatsu1), Hiroyuki Yamada1), Syota Murata2), Atsushi Yuki3), Shigeki Misawa4), Toyoko Oguri5), Satoshi Mitarai1) |
| Affiliation |
1) Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association 2) Department of Clinical Laboratory, Chiba University Hospital 3) Department of Laboratory Medicine, National Defense Medical College Hospital 4) Department of Clinical Laboratory, Juntendo University Hospital 5) Department of Laboratory Medicine, Kameda Medical Center |
| Publication | J.J.C.M.: 22 (4), 279-283, 2012 |
| Received | August 8, 2012 |
| Accepted | October 23, 2012 |
| Abstract | A panel testing was conducted to assess the laboratory performance of smear microscopy examination for the detection of acid-fast bacilli (AFB), and its feasibility. A total of 87 volunteer facilities, including 83 hospitals and 4 private laboratories, attended the external quality assessment (EQA) trial. The conventional Ziehl-Neelsen (Z-N) and fluorescent (i.e. auramine-O, auramine-rhodamine, or acridine orange) staining methods were used in 56 and 31 facilities, respectively. Two facilities reported the results of Z-N and fluorescent staining methods. Five unstained slides with known AFB smear positivity which were prepared using artificial sputum specimen were sent to each volunteer facility by mail, and the total of 435 reading results (100%) was reported. Comparing to the standard results, 405 (93.1%) results were correct, while 26 (6.0%), 2 (0.5%), 1 (0.2%) and 1 (0.2%) were low false negative, low false positive, high false positive and high false negative, respectively. Z-N was relatively less sensitive than fluorescent staining method in general. The EQA for AFB smear microscopy was implemented. It was relatively easy to conduct using standardized panel testing slides, and showed generally high quality of performance in the participating facilities. The activity could be further expanded to monitor the general quality of smear microscopy in Japan. |
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| Copyright © 2002 The Japanese Society for Clinical Microbiology All rights reserved. |