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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Analysis of Staphylococcus spp. inducible clindamycin resistance by microdilution method |
| Language |
J |
| AuthorList |
Kayoko Sano1), Kasumi Takizawa1), Takayuki Kurosawa1), Tomo Hirano1), Yasuyuki Sato1), Yoshimi Sato1), Midori Sumitomo1), Sigeru Ehara1), Shinitirou Watanabe1), Toshihiro Mituda2) |
| Affiliation |
1Department of Clinical Laboratory Medicine, Yokohama University Hospital
2Department of Infectious Diseases, Yokohama University Hospital |
| Publication |
J.J.C.M.: 23 (1), 12-19, 2013 |
| Received |
October 4, 2012 |
| Accepted |
January 16, 2013 |
| Abstract |
Since Clinical Laboratory Institute (CLSI) released M100-S18 in 2008, Staphylococcus spp. strains are recommended to be tested for inducible clindamycin resistance by D-zone test using Kirby-Bauer disks or microdilution method. For microdilution method to test inducible clindamycin-resistance, CLSI recommends the combination of erythromycin (EM): 4 μg/mL plus clindamycin (CLDM): 0.5 μg/mL in a well. We evaluated microdilution method for testing inducible clindamycin-resistance with domestic Staphylococcus spp. strains across a wide variety of concentration combinations. As a result, drug concentrations ranging between EM: 2-8 μg/mL with combinations of CLDM: 0.25-1 μg/mL were identified as optimal concentrations to detect inducible clindamycin-resistant strains (i.e., CLSI optimal concentration exist in the middle of each concentration range). |
| Keywords |
Staphylococcus spp., D-zone test, macrolide-lincosamide-streptogramin (MLSB) resistance, microdilution methodinducible clindamycin-resistant |
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