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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Usefulness of ACID PLUS decontamination procedure for MGIT |
| Language |
J |
| AuthorList |
Eri Hayakawa, Manabu Nakano |
| Affiliation |
Department of Clinical Laboratory, National Hospital Organization Mie-Chuo Medical Center |
| Publication |
J.J.C.M.: 23 (1), 20-24, 2013 |
| Received |
October 4, 2012 |
| Accepted |
January 28, 2013 |
| Abstract |
In order to reduce various-germs contamination, we used ACID PLUS (Kyokuto Pharmaceutical Industrial Co., Ltd.) decontamination procedure for Mycobacteria Growth Indicator Tube (MGIT, Becton Dickinson Japan). This decontamination procedure is the method which added ACID PLUS to the N-acetyl-L-cysteine (NALC)-NaOH processing method. We compared the NALC-NaOH and ACID PLUS decontamination procedures by using MGIT system. The positive ratio for acid-fast culture of NALC-NaOH and ACID PLUS were both 20.3% (130/640) and coincidence rate was 95.6% (612/640) (N.S.). The decontamination rate was 12.5% (80/640) in NALC-NaOH and 0.9% (6/640) in ACID PLUS. The mean time of the recovery of mycobacteria strains in the NALC-NaOH was 11-days and in the ACID PLUS was 14-days. As mentioned above, minimizing the influence of acid-fast-bacilli growth and reducing various-germs contamination sharply and efficient, it was suggested that this ACID PLUS decontamination procedure can be set to one of the useful pre-treating methods. |
| Keywords |
N-acetyl-L-cysteine, MGIT |
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