 |
The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
The usefulness of the LAMP method for diagnosing Mycoplasma infection |
| Language |
J |
| AuthorList |
Takahiro Kinebuchi1), Hiroki Kano1), Fujio Kakuya2), Ryousuke Tanaka2), Hiroaki Fujiyasu2) |
| Affiliation |
1Department of Clinical Laboratory, Social Welfare Corporation Hokkaido Social Work Association Furano Hospital
2Department of Pediatrics, Social Welfare Corporation Hokkaido Social Work Association Furano Hospital |
| Publication |
J.J.C.M.: 23 (2), 87-94, 2013 |
| Received |
January 8, 2013 |
| Accepted |
April 3, 2013 |
| Abstract |
In this paper, the usefulness of the loop-mediated isothermal amplification (LAMP) method for diagnosing Mycoplasma infection is examined. For this, a culture method, polymerase chain reaction (PCR), a serological-test, and macrolide antimicrobial resistance were used. In this study, 167 cases were analyzed in which a Mycoplasma infection was suspected. Pharyngeal specimens were used for the LAMP and the culture methods. For the serological-test, enzyme immunoassay (EIA) and particle agglutination (PA) were used. The LAMP method exhibited a sensitivity of 11.4% and the culture method and the consistent became a negative in the PCR method in one case. With regard to LAMP method positivity, 36.8% became EIA positive and 31.6% became positive by single-serum PA. Using PA, all 14 cases that were possible for paired-serum extraction were positive. Moreover, the resistance of the macrolide was 89.5%. Reliability evaluations of the LAMP method showed it has 95% sensitivity and 100% specificity, thus it has a high usefulness in diagnosing Mycoplasma infection. |
| Keywords |
Mycoplasma pneumoniae |
|
