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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Molecular Analysis of Macrolide-Nonsusceptible Moraxella catarrhalis |
| Language |
J |
| AuthorList |
Kageto Yamada1), Machiko Kashiwa1), Haruko Kazama2), Ryoichi Saito3) |
| Affiliation |
1) Department of Clinical Laboratory, Toshima Hospital
2) Department of Clinical Laboratory, Bokuto Hospital
3) Department of Microbiology and Immunology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University |
| Publication |
J.J.C.M.: 23 (2), 95-101, 2013 |
| Received |
August 27, 2012 |
| Accepted |
April 18, 2013 |
| Abstract |
Moraxella catarrhalis produces β-lactamase, is known to be a causative agent of pneumonia. Through basic analysis, here we report that we analyzed the mechanism of resistance in M.catarrhalis, and found M.catarrhalis showed high-level resistance to macrolide antibiotics. Drug susceptibility tests by disk diffusion with clarithromycin, erythromycin, clindamycin and amoxicillin/clavulanate (AMPC/CVA) showed high-level resistance to macrolide antibiotics with exception of AMPC/CVA. Although we further performed PCR to detect mefA, ermA, ermB and ermC gene, none of these genes were detected. Analysis of 23S rRNA gene mutation using PCR and sequencing revealed one point mutation in 23S rRNA (A2058T; Escherichia coli numbering) which is considered as a cause of high-level resistance to macrolide antibiotics. Due to predicted increase in high-level macrolide antibiotics resistant M.catarrhalis strains in the future, therefore, we need to focus on the rate of dispersion. |
| Keywords |
23S rRNA, Moraxella catarrhalis, clarithromycin |
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