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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of a commercial enzyme immunoassay test kit for detection of Clostridium difficile toxins A and B in stool specimens |
| Language |
J |
| AuthorList |
Mayumi Oki1), Shigeki Misawa1), Hiromi Arai1), Yukiko Kawase1), Masayoshi Chounan1), Takaaki Kawakami1), Yutaka Kuno1), Takashi Horii1), Shigemi Kondo2), Kazunori Miyake2), Yoko Tabe2), Akimichi Ohsaka1,3) |
| Affiliation |
1) Department of Clinical Laboratory, Juntendo University Hospital
2) Department of Clinical Laboratory Medicine, Juntendo University School of Medicine
3) Department of Transfusion Medicine and Stem Cell Regulation, Juntendo University School of Medicine |
| Publication |
J.J.C.M.: 23 (4), 259-263, 2013 |
| Received |
November 6, 2012 |
| Accepted |
October 24, 2013 |
| Abstract |
We evaluated the performance of detection of Clostridium difficile toxins A and B in stool specimens by a rapid enzyme immunoassay test kit, TOX A/B QUIK CHEK (TOX A/B), in comparison with toxigenic culture as the reference method. In addition, semiquantitative analysis of C. difficile culture was compared with the stool toxin detection by TOX A/B. A total of 232 diarrheal stool specimens were collected and assayed from patients suspected of having C. difficile infection (CDI). Of the 92 culture-positive specimens, PCR analysis revealed 55 toxin A- and toxin B-positive isolates, 11 toxin A-negative and toxin B-positive isolates, and 26 toxins A- and B-negative isolates. Therefore, 66 specimens showed positive toxigenic culture results; in comparison with the toxigenic culture method, the sensitivity and specificity of the TOX A/B test for detection of toxins A and B in stool specimens were 42.4% (28/66) and 98.8% (164/166), respectively. The results of toxin detection by TOX A/B were likely positive in the specimen with the greater number of colonies on semiquantitative culture plate. Because the TOX A/B test has a low sensitivity for toxin detection in stool samples, analysis using a combination of the glutamate dehydrogenase antigen test and culture as well as toxigenic culture should be performed in suspected CDI cases with negative results for stool toxins using rapid test kits during routine testing. |
| Keywords |
Clostridium difficile, TOX A/B QUIK CHEK |
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