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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Prevalence of Extended-Spectrum Beta-Lactamase- and Metallo-Beta-Lactamase-Producing Enterobacteriaceae in Stools |
| Language |
J |
| AuthorList |
Kohei Yoshikawa1), Junya Osagawa1), Miyoko Sonoda2), Yasuaki Shimatani1), Mami Takeda1), Yukiyasu Kinoshita1) |
| Affiliation |
1) Clinical Laboratory, National Hospital Organization Osaka Medical Center
2) Clinical Laboratory, National Hospital Organization Kyoto Medical Center |
| Publication |
J.J.C.M.: 24 (1), 9-16, 2014 |
| Received |
October 7, 2013 |
| Accepted |
January 29, 2014 |
| Abstract |
To grasp a situation of carriers with extended-spectrum beta-lactamase (ESBL)- and metallo-beta-lactamase (MBL)-producing organisms in the gastrointestinal tract, we conducted a survey on ESBL- and MBL-producing Enterobacteriaceae in 310 stool specimens collected in Osaka National Hospital. CHROMagar ESBL and CHROMagar KPC media were used as selective media for ESBL producers and MBL producers, respectively. Confirmatory tests were also performed on the isolates which grew on the selective media. As confirmatory tests, ESBL production and MBL production were determined by double disk synergy test with clavulanic acid/amoxicillin disk and sodium mercaptoacetic acid disk, respectively. ESBL-producing organisms were isolated from 37 samples (11.9%) including 45 strains; MBL-producing organisms from 11 samples (3.5%) including 14 strains. All of the 14 MBL-producers were also confirmed as positive ESBL. The 45 ESBL-positive strains included 20 strains of Escherichia coli, 12 strains of Klebsiella pneumoniae, 6 strains of Enterobacter cloacae, 3 strains of Klebsiella oxytoca, each strain of Enterobacter aerogenes, Citrobacter freundii, Proteus mirabilis, and Morganella morganii. The 14 MBL-positive strains included 7 strains of K. pneumoniae, 3 strains of E. cloacae, 2 strains of K. oxytoca, 1 strain of E. aerogenes, and 1 strain of E. coli. β-Lactamase genes were identified from the positive strains by PCR. 11 strains out of the 45 ESBL-producing strains contained CTX-M-2, while all of 14 MBL-producing strains contained IMP-1. Carriers with ESBL- and MBL-producing Enterobacteriaceae tended to be geriatric and inpatient with relatively long periods hospitalization. No underlying conditions were commonly found among the carriers with ESBL-producing organisms; however, some carriers with MBL-producing organisms tended to have malignancies. |
| Keywords |
ESBL, MBL, RAPD-PCR, active surveillance |
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