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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Carbon dioxide-dependent Escherichia coli isolated from a sepsis patient with cholangitis: a case report |
| Language |
J |
| AuthorList |
Masaru Takamizawa1), Takehisa Matsumoto2), Hironobu Nakamura3), Tatsuro Mitsuhashi3), Yuki Takamatsu1), Ryosuke Kato1), Katsuko Notake1), Masae Uehara1), Maki Omori1), Emi Yagasaki1), Kyoko Ide1) |
| Affiliation |
1) Department of Laboratory Medicine, Saku Central Hospital
2) Department of Laboratory Medicine, Shinshu University Hospital
3) Department of General Medicine, Saku Central Hospital |
| Publication |
J.J.C.M.: 24 (1), 29-35, 2014 |
| Received |
September 19, 2013 |
| Accepted |
November 28, 2013 |
| Abstract |
Small-colony variants (SCVs) constitute a slow-growing subpopulation of bacteria with distinctive phenotypic and pathogenic traits. Atypical colony morphology and altered biochemical profile may lead to failure in identification of SCV strains. We here report for the isolation of an Escherichia coli SCV phenotype from a sepsis patient with cholangitis. A gram-negative bacterium from blood culture was isolated in pure culture. Gram-negative, rod-shaped organisms failed to grow on BTB agar incubated in ambient air. On the other hand, the isolate grew on BTB agar under 5%CO2 gas condition. It was the typical features of a SCV. Matrix-assisted laser desorption ionization-time of flight mass spectrometry systems (Bruker Daltonics) and 16S rRNA sequencing identified the bacterium as Escherichia coli. We failed to identify the E. coli isolate or determine its antimicrobial susceptibilities using the MicroScan WalkAway 96SI (Siemens Healthcare Diagnostics) System because the isolate did not grow in the system. However, we were able to obtain results of identification and antimicrobial susceptibilities by under 5%CO2 gas condition. To our knowledge, this is the first clinical isolation of capnophilic E. coli in Japan. |
| Keywords |
Small-colony variants, Escherichia coli |
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