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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Clinical microbiological characterization of enterohemorrhagic Escherichia coli strains isolated from 1990 to 2012 |
| Language |
J |
| AuthorList |
Osamu Kobayashi1), Masahiro Isozaki2), Maki Kitagawa3), Hiroshi Enari3,4) |
| Affiliation |
1) Department of Clinical Laboratory, Noto General Hospital
2) Department of Clinical Laboratory, Amakusa Medical Center
3) Kyokuto Pharmaceutical Industrial Co., Ltd.
4) Division of Anaerobe Research, Life Science Research Center, Gifu University |
| Publication |
J.J.C.M.: 25 (1), 34-41, 2014 |
| Received |
December 9, 2013 |
| Accepted |
September 16, 2014 |
| Abstract |
We carried out a study for the detection of biochemical characterization and virulence genes (stx1, stx2, eaeA, aggR, bfpA, astA, hlyA) from forty-three strains of enterohemorrhagic Escherichia coli(EHEC). These strains were isolated from 1990 to 2012 and consisted of 13 serogroups (O1, O26, O91, O103, O111, O113, O121, O128, O145, O157, O165, O169, O177) and nontypable (2 strains). The number of strains possessing the following virulence genes were broken down as follows: stx1: 30, stx2: 29, eaeA: 36, aggR: 0, bfpA: 0, astA: 15, and hlyA: 42. Although 41 strains were confirmed to produce enterohemolysin, the remaining one strain could not be confirmed enterohemolysin by producing alpha-hemolysin on EHT agar. For the characterization of these forty-three (EHEC) isolates, we assayed for the following: lysine decarboxylase, indole production, β-D-galactosidase, β-D-glucuronidase, sorbitol fermentation, shiga toxin typing, confirmation test of enterohmoysin, and cefixime tellurite (CT) sensitivity test. In our study, fourteen strains of EHEC did not grow on CT-SMAC (CT-RMAC) agar plates. Among the fourteen strains, O157 and O26 of two strains each were included. For even targeting EHEC O26 or EHEC O157, a screening plate without CT must also be used. There were several atypical EHEC strains that could not be detected even using multiple media. A new selective media for detection of EHEC is required. |
| Keywords |
enterohemolysin |
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