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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of RAPIRAN S. pneumoniae HS test for the detection of pneumococcal antigen in middle ear fluids from children with acute otitis media |
| Language |
J |
| AuthorList |
Junko Masaki, Iku Kurokawa, Katsunori Takashima |
| Affiliation |
Department of Laboratory, Medicine Tohoku Rosai Hospital Japan Labor Health & Welfare Organization |
| Publication |
J.J.C.M.: 25 (2), 99-105, 2015 |
| Received |
June 30, 2014 |
| Accepted |
October 28, 2014 |
| Abstract |
RAPIRAN S. pneumoniae HS (HS test) is an immunochromatographic test to detect pneumococcal antigen in middle ear fluids and nasopharyngeal secretion from otitis media and rhinosinusitis patients. We evaluated HS test to detect pneumococcal antigens in 135 samples obtained from pediatric acute otitis media patients (average age: 1.3, age range: 0-8) from December 2011 to December 2012. All samples were subjected to conventional bacterial cultures, Gram staining and HS test measurement. Streptococcus pneumoniae was detected in 14 (10.4%) of the 135 samples, 29 (21.5%) were detected as Streptococcus spp. by Gram staining, and 20 (14.8%) were positive by the HS test. The positive ratio, negative ratio, positive predictive value (PPV), and negative predictive value (NPV) based on the bacterial culture was 71.4%, 84.3%, 34.8%, and 96.2%, respectively, by Gram staining. On the other hand, the positive ratio, negative ratio, PPV, and NPV by the HS test was 64.3%, 90.9%, 45.0%, and 95.7%, respectively. We can obtain valuable information for severity of inflammation by the counts of neutrophils and for estimation of pathogens by Gram staining. And the HS test exhibited comparable detection ability. In adition, HS test does not require specific techniques and equipment to identify pathogens and results can be obteined within 30 minites. In conclusion, the HS test may be a useful method for relevant selection of antimicrobial drugs even in an insufficient facility. |
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