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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of the PURE-LAMP method for diagnosing Mycobacterium tuberculosis complex |
| Language |
J |
| AuthorList |
Masahiro Kobayashi1), Sadao Aoki1), Katuhito Koike1), Ataru moriya2), Minoru Katou1), Takefumi Saito3) |
| Affiliation |
1) Department of Clinical Laboratory, National Hospital Organization Ibarakihigashi National Hospital
2) Department of Clinical Laboratory, National Hospital Organization Disaster Medical Center
3) Department of Respiratory Medicine, National Hospital Organization Ibarakihigashi National Hospital |
| Publication |
J.J.C.M.: 25 (2), 106-110, 2015 |
| Received |
September 1, 2014 |
| Accepted |
November 11, 2014 |
| Abstract |
Nucleic acid amplification (NAA)-test is important tool for diagnosing Mycobacterium tuberculosis complex (MTB). We investigated new NAA-test using the Procedure for Ultra Rapid Extraction (PURE)-Loop-mediated isothermal amplification (LAMP) method. This study was used only sputum and compared smear-test, culture-test, identification-test and other NAA-test. The LAMP method and other NAA-test exhibited a sensitivity of 84%.There are showed the same result on 159 specimens from a total of 161 sputum specimens. A sensitivity of smear-positivity and smear negative were 100% and 73% together with the LAMP method and other NAA-test. Amplification inhibition of the LAMP method was not considered from positive control, negative control and culture-test result. From the above, it was considered that the LAMP method was equivalent to the conventional NAA-test. |
| Keywords |
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