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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of streptococci |
| Language |
J |
| AuthorList |
Yusuke Ota1), Takehisa Matsumoto2), Eriko Kasuga2), Kazuki Horiuchi2), Tatsuya Negishi2), Tomomi Yaguchi2), Tatsuya Natori2) |
| Affiliation |
1) Department of Health and Medical Sciences, Shinshu University, Nagano, Japan
2) Department of Laboratory Medicine, Shinshu University Hospital, Nagano, Japan |
| Publication |
J.J.C.M.: 25 (2), 111-116, 2015 |
| Received |
October 1, 2014 |
| Accepted |
December 2, 2014 |
| Abstract |
The performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry systems were evaluated in the identification of streptococci. A total of 135 clinical isolates were analyzed using a Bruker MALDI Biotyper system. This system correctly identified 100% (135/135) of Streptococcus isolates to the genus-level and 81.5% (110/135) to the species-level for two sample preparation methods (on-plate formic acid preparation and ethanol-formic acid extraction). Several bacterial species could not be identified to the species-level by this system because of few number of reference spectra in current reference library. It is necessary to improve reference library in MALDI Biotyper for the more accurate identification of clinically significant Streptococcus strains. |
| Keywords |
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