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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
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[Vol.25 No.3 contents] Japanese / English |
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| ArticleTitle | A study using a Real-time PCR to determine optimal specimen collection sites for detecting Mycoplasma pneumoniae antigen by a rapid immunochromatographic assay kit |
| Language | J |
| AuthorList | Yasushi Shimada1), Atsuko Minagawa1), Yumiko Hirayama1), Kazuyuki Sugiyama1), Hatsue Itagaki1), Masaki Ito2), Aya Takeyama2), Youichi Tomita2), Hiroko Sakuma3), Keiko Mitamura4), Tomohiro Ooishi5), Tadashi Saito6), Michiyoshi Minato7), Yuuki Tsukahara8), Mitsuaki Hosoya9) |
| Affiliation |
1) Research & Development/Clinical Development, LSI Medience Corporation 2) Department of Pediatrics, Soma General Hospital 3) Department of Pediatrics, Hoshi General Hospital 4) Department of Pediatrics, Eiju General Hospital 5) Department of Pediatrics, Niigata University Medical & Dental Hospital 6) Department of Pediatrics, Tako Central Hospital 7) Minato Pediatric Clinic 8) Shinmei Kodomo Clinic 9) Department of Pediatrics, Fukushima Medical University School of Medicine |
| Publication | J.J.C.M.: 25 (3), 196-203, 2015 |
| Received | October 7, 2014 |
| Accepted | January 21, 2015 |
| Abstract | To consider the optimal specimen collection site in the pharyngeal region when using a rapid immunochromatographic assay kit (Prorast Myco) to detect Mycoplasma pneumoniae antigen, we quantified the number of M. pneumoniae genes obtained or prepared from the posterior pharyngeal wall and palatine tonsil collection sites of patients by real-time PCR. Comparison of the number of M. pneumoniae genes of the same patient group (N=25) positive for M. pneumoniae by nested PCR showed a trend toward a higher number of genes obtained from posterior pharyngeal wall specimens than from palatine tonsil specimens. Next, comparison of the number of genes obtained from the posterior pharyngeal wall collection group (N=44) with those from the palatine tonsil collection group (N=35) showed significantly higher concentrations of M. pneumonia genes in the posterior pharyngeal wall collection group (1.0×105-2.7×108 copies/mL) compared with the palatine tonsil collection group (6.8×103-1.5×107 copies/mL) (p<0.01). In addition, the positive concordance rates between Prorast Myco and PCR of the posterior pharyngeal wall collection group and the palatine tonsil collection group were 79.5% and 22.9%, respectively. The positive concordance rate of the posterior pharyngeal wall collection group was significantly higher than that of the palatine tonsil collection group. For the detection of M. pneumoniae antigen using Prorast Myco, the optimal specimen collection site appears to be the posterior pharyngeal wall. |
| Keywords | M. pneumoniae, Real-time PCR |
| Copyright © 2002 The Japanese Society for Clinical Microbiology All rights reserved. |