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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
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[Vol.25 No.3 contents] Japanese / English |
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| ArticleTitle | Reduced coagulase production in MRSA isolated from blood cultures during antimicrobial treatment |
| Language | J |
| AuthorList | Michiko Furugaito1), Yoshiki Misawa3), Atsushi Yoshida4, 5), Katsuko Okuzumi4), Yuko Tahara1), Hirofumi Toda1), Koji Utsunomiya1), Hiromi Matsuura1), Toshinori Kamisako2) |
| Affiliation |
1) Department of Clinical Laboratory, Kinki University Hospital 2) Department of Clinical Laboratory Medicine, Kinki University School of Medicine 3) Department of Infection Control and Prevention, the University of Tokyo Hospital 4) Division of Infection Control, Dokkyo Medical University Hospital 5) Department of Clinical Laboratory Medicine, Dokkyo Medical University Hospital |
| Publication | J.J.C.M.: 25 (3), 204-212, 2015 |
| Received | October 2, 2014 |
| Accepted | February 10, 2015 |
| Abstract | We isolated methicillin-resistant Staphylococcus aureus (MRSA) from blood cultures of a 66-year-old man who had necrosis in his right lower extremity, before and during antimicrobial treatment. Both of the isolated MRSA were bound coagulase negative (RABBIT PLASMA 'Eiken'), whereas the tube coagulase test (free coagulase) was only positive in MRSA isolated before antimicrobial treatment, suggesting diminished/reduced coagulase production under the treatment. As for the MRSA under treatment, the tube coagulase test with RABBIT PLASMA 'Eiken' which dissolved in PEARLCORE TRYPTO-SOY BROTH 'Eiken' showed positive after 24 hours. Coagulase titer was 2 MCD/hr for the MRSA pretreatment and <1 MCD/hr for the MRSA under treatment. With the latex reagent, both MRSA were negative on PS LATEX 'Eiken' and STAPHYLO LA "SEIKEN" whereas both were positive on PASTOREX STAPH PLUS and DRYSPOT STAPHYTECT PLUS. Both of the MRSA were identical in POT (Phage Open-reading Frames Typing) method 93-217-107, SCCmec type IIa, and biochemical characteristics except coagulase production. This is the first report that free coagulase reaction became negative against MRSA isolated during antimicrobial treatment. We should be careful not to misidentify MRSA with diminished/reduced coagulase production as coagulase-negative staphylococci. |
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