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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
A study on identification of Acinetobacter species isolated from blood culture |
| Language |
J |
| AuthorList |
Yuka Azuma, Tomohisa Watari, Yuki Shibazaki, Takatoshi Yonezawa, Shiori Yamauchi, Yutaka Tomoda, Satoshi Fujii |
| Affiliation |
Department of Medical Laboratory and Blood Center, Asahikawa Medical University Hospital |
| Publication |
J.J.C.M.: 25 (3), 213-222, 2015 |
| Received |
December 5, 2014 |
| Accepted |
March 5, 2015 |
| Abstract |
Acinetobacter species include many strains, such as A. baumannii, A. nosocomialis, A. pittii, A. lwoffii and A. radioresistens. In our routine identification methods, it is considerably difficult to identify all of the species because of their bacteriological similarities. It is particularly difficult to distinguish A. baumannii complex, which consists of A. baumannii, A. nosocomialis, A. pittii and A. calcoaceticus. In this study, 35 Acinetobacter isolates from blood culture were examined using conventional identification methods such as Gram strain, growth capability on the MacConkey agar and Drigalski agar, MALDI-TOF-MS (VITEK MS) and molecular biological method (rpoB gene sequence analysis and 16SrRNA gene sequence analysis). The species consisted of A. baumannii complex (80%) and non-A. baumannii complex (20%). A. nosocomialis (50%) was the most prevalent species in the A. baumannii complex, followed by A. baumannii (21.4%), and A. pittii (21.4%). The coincidence rate based on rpoB gene sequence was 77.1% (E/NF), 62.9% (NID), 88.6% (GN) and 91.4% (MS). Because severity of illness score (pitt bacteremia score) of A. baumannii complex was significantly higher than that of non-A. baumannii complex, it is important to exactly distinguish A. baumannii complex from non-A. baumannii complex. There was no difference in severity of illness score among A. baumannii complex. VITEK MS was useful for distinguishing A. baumannii complex from non-A. baumannii complex. |
| Keywords |
Acinetobacter, Acinetobacter baumannii complex |
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