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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Corneal ulcer caused by Moraxella nonliquefaciens |
| Language |
J |
| AuthorList |
Tatsuya Ohno1), Yosuke Tanaka1), Momoko Anzai1), Nao Komatsu1), Tadatomo Ohyanagi2), Hironobu Akita3) |
| Affiliation |
1) Department of Clinical Laboratory, St. Marianna University School of Medicine Yokohama City Seibu Hospital
2) Department of Clinical Laboratory, St. Marianna University School of Medicine
3) Department of Pediatrics, St. Marianna University School of Medicine Yokohama City Seibu Hospital |
| Publication |
J.J.C.M.: 25 (3), 234-240, 2015 |
| Received |
November 12, 2014 |
| Accepted |
February 19, 2015 |
| Abstract |
We report a case of corneal ulcer caused by Moraxella nonliquefaciens in an 81-year-old man with diabetes mellitus. M. nonliquefaciens was isolated from the corneal scrapings of the patient. Gram-stained smears of the corneal scrapings showed numerous polymorphonuclear leukocytes and confirmed the presence of gram-negative bacilli arranged in pairs. When grown on 5% sheep blood agar for 24 h, M. nonliquefaciens formed colonies that appeared translucent, glistening, and spreading, with diameters ranging between 1.0 and 2.0 mm. However, identification cannot be based only on colony morphology because it changes depending on the culture medium used and is different among the strains of M. nonliquefaciens. Moreover, a Moraxella catarrhalis strain is known to have morphology similar to that of M. nonliquefaciens. Therefore, detection of this organism in respiratory-tract clinical samples requires caution. Biochemical tests are not sufficient to confirm the identity of this organism. However, 16S rRNA gene sequencing and mass spectrometry successfully identified the isolates. The etiological agents of corneal ulcer have changed overtime. Therefore, it is important to accurately identify this organism and carry out antimicrobial susceptibility tests. |
| Keywords |
Moraxella nonliquefaciens, spreading colony |
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