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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Detection of Group B Streptococci with Reduced β-lactam agents Susceptibility |
| Language |
J |
| AuthorList |
Mari Kusuki1), Tatsuya Nakamura1,2), Saori Kobayashi1), Yasuna Kobayashi1), Kenichiro Ohnuma1), Miyuki Yano1), Takumi Jikimoto1,2), Masakuni Nakamura1), Nobuhide Hayashi1), Kouji Kimura3), Yoshichika Arakawa3), Hiroyuki Yoshida2), Soichi Arakawa2) |
| Affiliation |
1) Department of Clinical Laboratory, Kobe University Hospital
2) Department of Infection Control and Prevention, Kobe University Hospital
3) Department of Bacteriology, Nagoya University Graduate School of Medicine |
| Publication |
J.J.C.M.: 25 (4), 283-289, 2015 |
| Received |
January 30, 2015 |
| Accepted |
May 7, 2015 |
| Abstract |
Streptococcus agalactiae (group B streptococci: GBS) causes serious infections in neonates, and elderly people. Penicillins are the first-line drug for the treatment of GBS infections, since all clinically isolated GBS have been considered uniformly susceptible to β-lactams, including penicillins. Recently, GBS isolates with reduced penicillin susceptibility (PRGBS) were reported, then we investigated the PRGBS in clinical specimens of our hospital. There was no PRGBS among 72 GBS isolates, however, 33 GBS isolates formed colonies in the growth-inhibition zone around ceftibuten disk. We sequenced PBP2X genes of 6 GBS isolates randomly chosen from these 33 isolates. Amino acid substitutions, S353Y, I373N, T394I and F399V were found in their PBP2X. These substitutions are identical or found in neighboring positions to those reported in PBP2X of PRGBS, though neither V405A nor Q557E, key substitutions of PRGBS, was found. In this study, we found the amino acid substitutions of PBP in GBS are diverse, and this observation may indicate the presence of prodromal stage for PRGBS. |
| Keywords |
PRGBS, PBP2X, ceftibuten disk, β-lactam |
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