 |
 |
 |
The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
|
[Vol.25 No.4 contents] Japanese / English |
|
| ArticleTitle | Molecular characterization of non-carbapenemase-producing carbapenem-resistant Klebsiella pneumoniae sequence type 37 clinical isolates from nosocomial infections |
| Language | J |
| AuthorList | Takahiro Harada1), Masami Toyama2,3), Yukiko Nagano3,4), Yoshichika Arakawa4), Noriyuki Nagano3,5) |
| Affiliation |
1) Clinical Microbiology Laboratory, National Hospital Organization Chiba Medical Center 2) Medical Microbiology Laboratory, Funabashi Municipal Medical Center 3) Department of Bacteriology II, National Institute of Infectious Diseases 4) Department of Bacteriology, Nagoya University Graduate School of Medicine 5) Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine |
| Publication | J.J.C.M.: 25 (4), 320-328, 2015 |
| Received | March 25, 2015 |
| Accepted | June 29, 2015 |
| Abstract | Multidrug-resistant Klebsiella pneumoniae strains Kp1 and Kp2, which was resistant to all β-lactams including carbapenems and other classes of antimicrobials, and K. pneumoniae strain Kp3 exhibiting similar susceptibility profiles except for carbapenem susceptibility were recovered from three patients on the same hospital unit during the five month December 2012 to April 2013 period. The three patients had overlapping periods of hospitalization, thus suggesting nosocomial transmission of those K. pneumoniae strains during hospital stay. PCR and sequencing analysis showed the presence of the DHA-1 plasmid-mediated AmpC β-lactamase genes in all strains. They also harbored plasmid-mediated quinolone resistance determinant qnrB as well as 16SrRNA methylase gene armA. K. pneumoniae strains Kp1, Kp2, and Kp3 with indistinguishable PFGE profiles belonged to the same MLST sequence type 37. Our results demonstrated likely transmission of multidrug-resistant K. pneumoniae strains in the unit. Carbapenem resistance in K. pneumoniae strains Kp1 and Kp2 may be due to expression of DHA-1 AmpC β-lactamase combined with the loss and/or decrease in outer membrane proteins because any carbapenemase genes including KPC type were not detected from them. DHA-1-producing K. pneumoniae was not detected by active surveillance cultures of patients and healthcare personnel started at the time of the third case. Also, strict contact precautions have been implemented, and the new patients harboring those organisms have not been identified so far. |
| Keywords | K. pneumoniae, ST37, DHA-1 |
| Copyright © 2002 The Japanese Society for Clinical Microbiology All rights reserved. |