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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
A case of Helicobacter fennelliae bacteremia diagnosed by 16S rDNA using directly-obtained positive blood culture suspension |
| Language |
J |
| AuthorList |
Chikako Maehara1), Sadako Yoshizawa2), Yuto Fukui2), Kotaro Aoki3), Ayumi Yoshizumi3), Hinako Murakami1), Kumiko Yasui1), Shigeru Fukuzawa1), Kyoko Enokizono1), Waka Imai1), Masakazu Sasaki1), Yoshikazu Ishii3), Kazuhiro Tateda1,3), Kento Yanagisawa4), Ken Sakai4), Atsushi Aikawa4) |
| Affiliation |
1) Department of Laboratory Medicine, Toho University Omori Medical Center
2) Department of Infectious Diseases, Toho University Omori Medical Center
3) Department of Microbiology and Infectious Diseases, Toho University School of Medicine
4) Department of Nephrology, Toho University Omori Medical Center |
| Publication |
J.J.C.M.: 26 (1), 47-53, 2015 |
| Received |
May 21, 2015 |
| Accepted |
August 19, 2015 |
| Abstract |
A 50-years-old man receiving a living-donor kidney transplantation due to polycystic kidney was admitted to the hospital due to fever, chilling, and upper abdominal pain. Two sets of blood cultures obtained on admission revealed spiral-shaped gram negative rods on hospital day number six. Film-like colony was observed on hospital day number eleven and biochemical characteristics showed possible Helicobacter species, which was identified as H. fennelliae by using api-Helico®. To identify the bacteria earlier, 16S rDNA analysis was performed by using positive blood culture suspension directly. The results revealed 99.87% homology with H. fennelliae, which was compatible to the biochemical results. MIC values of isolated bacteria were 8 μg/mL for cefpirome, 8 μg/mL for levofloxacin, and 0.25 μg/mL for meropenem, respectively. Our results indicated possible beneficial role of 16S rDNA analysis using positive blood culture suspension directly. It may enable to identify bacteria such as Helicobacter species, much earlier than biochemical results. |
| Keywords |
Helicobacter fennelliae, 16S rDNA |
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