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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
A new trend in clinical microbial studies using MALDI-TOF MS From principle to application |
| Language |
J |
| AuthorList |
Masaru Komatsu |
| Affiliation |
Department of Clinical Laboratory Science, Faculty of Health Care, Tenri Health Care University |
| Publication |
J.J.C.M.: 26 (2), 79-89, 2016 |
| Received |
February 9, 2016 |
| Accepted |
|
| Abstract |
Almost 10 years have passed since the method of identifying bacteria using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) first appeared in the field of clinical microbiology. In Japan, more and more institutions are using this method every year, and the turn-around time of microbial studies in general is being reviewed. MALDI-TOF MS is drawing attention as a rapid method of identification because if there are isolated colonies, about 10 minutes are needed to complete the measurement using simple procedures. Furthermore, developments in the method of preprocessing colonies and enhancements of the database have contributed to the improved accuracy of identification of anaerobic bacteria, Mycobacterium, Nocardia and fungi. Bacteria that exist in fluid specimens such as spinal fluid, urine or blood culture fluid can be directly identified by using a 30 to 60-minute preprocessing method to remove contaminating proteins. A new β-lactamase examination method has been invented in which after mixing a solution of antibacterial agent with the bacterial culture, variations in molecular weight that are associated with the structural mutation of a β-lactam agent via hydrolysis are detected by MALDI-TOF MS. Also, multivariate analysis conducted with software of the mass spectra of clinically important resistant bacteria or toxin-producing strains such as extended-spectrum β-lactamase-producing bacteria, methicillin-resistant Staphylococcus aureus and verotoxin-producing Escherichia coli has received attention as an applicable method for the discovery and epidemiological analysis of biomarkers unique to bacteria. |
| Keywords |
MALDI-TOF MS |
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