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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Examination of the culture medium and susceptibility testing method of Mycoplasma pneumoniae |
| Language |
J |
| AuthorList |
Rumi Okuno1), Hiroaki Kubota1), Yumi Uchitani1), Hitomi Ohya2), Akihiko Hirai1), Akemi Kai1), Kenji Sadamasu1) |
| Affiliation |
1) Division of Microbiology, Tokyo Metropolitan Institute of Public Health
2) Division of Microbiology, Kanagawa Prefectural Institute of Public Health |
| Publication |
J.J.C.M.: 26 (2), 90-96, 2016 |
| Received |
June 23, 2015 |
| Accepted |
November 11, 2015 |
| Abstract |
Mycoplasma pneumoniae is one of the major causative bacteria for pneumonia, bronchitis and other community-acquired respiratory tract infections. Recently, an increase in the incidence of macrolide-resistant M. pneumoniae was reported in China, Korea and Japan. On the other hand, it is difficult to isolate M. pneumoniae because of contamination by other bacteria. We sometimes encountered such cases in which M. pneumoniae was not isolated using PPLO broth or Two-layer culture media, in spite of the detection of M. pneumoniae genes by PCR. Therefore, a modified culture medium with VCM was improved for the isolation of M. pneumoniae from the swabs of patients' pharynxes. M. pneumoniae was cultured from 68% out of swabs in the pl gene of M. pneumoniae-positive samples, when an improved medium was used. Antimicrobial susceptibility testing was performed using two types of broth micro-dilution methods. We compared the MICs of using the dry-plate (EIKEN Chemical Co., Ltd.) with the conventional method. No difference had observed in the MICs of the five M. pneumoniae strains including the standard strain. The improved culture medium and simplify antimicrobial susceptibility testing for M. pneumoniae, were very useful at laboratories for the investigation of M. pneumoniae. |
| Keywords |
Mycoplasma pneumoniae |
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