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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of MALDI-TOF MS analysis for the identification of rare nontuberculous mycobacteria species from clinical isolates |
| Language |
J |
| AuthorList |
Hiromichi Suzuki1), Atsushi Yoshida1,2,3), Tomomi Tarukawa1), Yuki Okamoto1), Kana Watanabe1), Yuuki Kobayashi1), Michiharu Asada1), Katsuko Okuzumi3), Atsuhito Fukusima1,2,3), Shinji Oikawa1), Yuko Kazumi4), Akira Hishinuma1,2) |
| Affiliation |
1) Clinical Laboratory Center, Dokkyo Medical University Hospital
2) Department of Infection Control and Clinical Laboratory of Medicine, Dokkyo Medical University
3) Division of Infection Control, Dokkyo Medical University Hospital
4) Department of Mycobacterium Reference and Research, the Research Institute of Tuberculosis |
| Publication |
J.J.C.M.: 26 (2), 105-111, 2016 |
| Received |
October 21, 2015 |
| Accepted |
January 6, 2016 |
| Abstract |
MALDI-TOF MS was evaluated for the identification of relatively rare species of nontuberculous mycobacteria (NTM) isolates. Fifty NTM clinical isolates (37 rapid growers and 13 slow growers) were analyzed by MALDI-TOF MS (Bruker Daltonics) as well as gene sequencing of 16S rRNA, hsp65 and rpoB. MALDI-TOF MS correctly identified 96.0% (48/50) of NTM isolates to the genus-level and 84.0% (42/50) to the species-level. Only two isolates of M. paraffinicum and M. wolinskyi (4%) could not be determined. Our study suggests that relatively rare NTM species can be reliably identified by MALDI-TOF MS and that its application in clinical setting is useful for selecting appropriate antimicrobial therapies. |
| Keywords |
MALDI-TOF MS |
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