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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Identification of an atypical strain of Vibrio cholerae |
| Language |
J |
| AuthorList |
Emi Sugano1), Yoshimi Morishita1), Yurika Nakajima1), Yuko Tateishi1), Haruka Kotake1), Kazuhisa Ugajin1), Sachiko Tahara1), Katsuhiko Yoshida1), Kunihiko Fukuchi2) |
| Affiliation |
1) Department of Clinical Laboratory, Showa University Hospital
2) Department of Pathology, Showa University Hospital |
| Publication |
J.J.C.M.: 26 (2), 119-123, 2016 |
| Received |
October 9, 2015 |
| Accepted |
November 10, 2015 |
| Abstract |
We identified a strain of Vibrio cholerae that showed atypical characteristics. This strain was isolated from a 54-year-old male who presented to the Emergency department of our hospital with muscle spasms throughout his entire body. The patient had watery diarrhea and nausea after returning from a 3-month trip throughout Asia. Stool cultures revealed that a significant number of non-lactose fermenting Gram-negative rods grew on a Drigalski plate, but not on a TCBS agar plate. The biochemical and physiological characteristics of the isolate were as follows; oxidase production: positive, Triple sugar iron agar: positive/positive, gas production from glucose: positive, hydrogen sulfide production: negative, indole production: positive, motility: positive, indolepyruvate production: negative, citrate utilization: negative, Voges-Proskauer test: positive, lysine decarboxylase: positive, and DNase: positive. The isolate was identified as V. cholerae by Walkaway96 with an identification probability of 94.9% and by api with 99.9%. The DNA sequence of the 16S rRNA region was 99% identical to that of the ATCC14035 strain of V. cholerae. The strain agglutinated with anti-V. cholerae O group 139 and was positive for the cholera toxin gene. Thus, we identified the strain as V. cholerae O139. |
| Keywords |
Vibrio cholerae, O139, TCBS |
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