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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Genome analysis of Moraxella catarrhalis and Moraxella nonliquefaciens exhibiting high resistance to macrolides, isolated from pediatric clinical specimens at a medical institution in Akita Prefecture, Japan |
| Language |
J |
| AuthorList |
Keiko Sato1), Jun Yatuyanagi2), Gene Igawa3), Ryoichi Saito3) |
| Affiliation |
1) Department of Clinical Laboratory, Akita Red Cross Hospital
2) Department of Bacteriology, Akita Prefectural Institute of Public Health
3) Department of Microbiology and Immunology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University |
| Publication |
J.J.C.M.: 27 (1), 8-14, 2016 |
| Received |
July 22, 2015 |
| Accepted |
July 19, 2016 |
| Abstract |
Moraxella catarrhalis is a causative agent of upper or lower respiratory inflammation and otitis media in children. In contrast, Moraxella nonliquefaciens is a normal inhabitant of the upper respiratory tract and is considered minimally pathogenic. However, it is isolated at a high rate from the upper respiratory tract of children with viral infections, and its genome reportedly contains genes encoding proteins involved in pathogenicity. In the present study, we investigated the resistance mechanism of M.catarrhalis and M.nonliquefaciens strains with high resistance to macrolides that were isolated from pediatric clinical specimens at a medical institution in Akita prefecture, Japan. All three isolated strains of M.catarrhalis had an A2058T mutation in Alleles 1 to 4 of the 23S rRNA gene. All three strains of M.nonliquefaciens also had an A2058T mutation in the 23S rRNA gene. None of the 6 strains had ermA, ermB, ermC, or mefA genes. Those results suggest that M.catarrhalis and M.nonliquefaciens, which acquired high resistance to macrolides via an A2058T mutation in the 23S rRNA gene, are prevalent in Japan. It is necessary to establish nation-wide surveillance system for monitoring prevalence of both these macrolide-resistant species in Japan. |
| Keywords |
Moraxella catarrhalis, Moraxella nonliquefaciens |
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