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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Gram staining using a modified Brun-Buisson method is useful for the diagnosis of catheter-related bloodstream infections |
| Language |
J |
| AuthorList |
Yumiko Tanimichi1), Michiko Yagoshi2), Mitsuru Yanai3), Tomohiro Nakayama1,4) |
| Affiliation |
1) Clinical Laboratory, Nihon University Itabashi Hospital
2) Department of Infection Prevention, Nihon University Itabashi Hospital
3) Division of General Medicine, Department of Internal Medicine, Nihon University School of Medicine
4) Division of Laboratory Medicine, Department of Pathology of Microbiology, Nihon University School of Medicine |
| Publication |
J.J.C.M.: 27 (4), 289-297, 2017 |
| Received |
November 15, 2016 |
| Accepted |
June 29, 2017 |
| Abstract |
For the purpose of the early diagnosis of catheter-related bloodstream infections (CRBSI), we routinely perform Gram staining of a suspension of indwelling intravascular catheter tip samples. To evaluate the usefulness of this test, we investigated 1,976 specimens submitted to the microbiology laboratory during a 5-year period between June 2006 and May 2011. Among 790 catheter specimens in which microorganisms were detected from the cultures of catheter tips, 34.2% tested positive with Gram staining. Among 419 cases in which the microorganisms isolated from the catheter cultures were the same as those identified from blood cultures, 50.8% of the microorganisms were detected by Gram staining. However, among 371 cases of colonization (positive for cultures of catheter tips and negative for blood cultures), only 15.4% of the microorganisms were detected by Gram staining. These results indicate the high sensitivity of Gram staining of catheter tips for detecting the causative microorganisms of CRBSI. To examine whether the result of smear-positive catheter tips is useful for the diagnosis of CRBSI, we reviewed the medical records of 109 cases in which the results of the Gram staining of catheter tips were reported before the blood cultures became positive. In 21 cases, the physicians started or adjusted antibacterial treatment on the day of the report based on the presumptive findings of Gram staining. In conclusion, Gram staining of a suspension of indwelling intravascular catheter tip is useful for the early diagnosis of CRBSI. |
| Keywords |
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