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Vol.33 No.5 contents | Japanese/English |
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Full Text of PDF (514K) Article in Japanese |
Horizontal Transmission Estimation of Methicillin-resistant Staphylococcus aureus Using Polymerase Chain Reaction-based Open Reading Frame Typing at a Children's Hospital
Chikako INAOKA1)5), Kuniko SHIRAO1)5), Futoshi FUJIWARA1)5), Makie KINOSHITA2)5), Takeshi HATACHI3)5) and Narutaka MOCHIZUKI4)5)
1)Clinical Laboratory Center, Osaka Women's and Children's Hospital, 2)Infection Control Office, Osaka Women's and Children's Hospital, 3)Department of Intensive Care Medicine, Osaka Women's and Children's Hospital, 4)Department of Neonatal Medicine, Osaka Women's and Children's Hospital, 5)Infection Control Team, Osaka Women's and Children's Hospital
Introduction: In this study, we used polymerase chain reaction-based open reading frame typing (POT) to estimate the horizontal transmission pathway for methicillin-resistant Staphylococcus aureus (MRSA) detected in routine nasal surveillance culture in neonatal and pediatric wards.
Methods: The horizontal transmission pathway for MRSA cases detected at Osaka Women's and Children's Hospital during July 2015-July 2016 was estimated using POT and antibiotic resistance patterns.
Results: Of the hospitalized patients with MRSA, 81 were classified as "in-hospital acquired," and of these patients, 33 shared a history of hospitalization in the same ward during the same period (7 POT types), thereby indicating horizontal transmission. In the neonatal ward, of the 42 patients with MRSA, the number of horizontal transmission cases was 3 (22 patients), whereas in the pediatric cardiovascular ward, the number of horizontal transmission cases was 4 (11 patients). The antibiotic resistance patterns in similar POT types were consistent with all strains in the case of horizontal transmission. None of the MRSA POT type obtained from nasal cavity culture of the ward staff at the onset of the outbreak matched with the POT type of patient-derived MRSA samples.
Conclusions: We could distinguish multiple cases of horizontal transmission at similar durations in the same ward using POT. Therefore, this method can be useful for understanding the horizontal transmission of MRSA within a ward.
Key words：MRSA, POT method, surveillance culture, antibiotic resistance pattern, horizontal transmission
e-mail: saikin＠wch.opho.jp
Received: December 9, 2017
Accepted: July 11, 2018
33 (5)：236─245,2018
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